Biomedical device having crosslinked biopolymer micro pattern and preparation thereof
AssigneeTamkang University
InventorsLung-Jieh Yang, Yu-Cheng Ou
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Abstract
This invention proposes a novel technique for fabricating a gelatin micro pattern for cell culture. The gelatin micro pattern is formed by photolithography and then crosslinked with a crosslinking agent such as glutaraldehyde. The gelatin micro pattern can be used as an excellent cell culture platform for in-vitro observations of a certain cluster of living cells or even a single living cell.
Claims19
A biomedical device having a crosslinked biopolymer micro pattern comprising a substrate and a crosslinked biopolymer micro pattern attached on the substrate.
A method for preparing a biomedical device having a crosslinked biopolymer micro pattern, which comprises the following steps:
- a) coating a substrate with a layer of biopolymer;
- b) coating a layer of photoresist on the biopolymer layer;
- c) imagewise exposing the photoresist layer;
- d) developing the exposed photoresist resulting from step c) to form a patterned photoresist layer, so that a portion of the biopolymer layer is exposed;
- e) contacting the exposed portion of the biopolymer layer with an aqueous solution containing a crosslinking agent, so that the exposed biopolymer is crosslinked;
- f) removing the patterned photoresist layer from the biopolymer layer;
- and
- g) immersing the resulting intermediate from step f) in water or an aqueous solution to remove another portion of biopolymer layer that has not been crosslinked, so that the substrate is formed with a crosslinked biopolymer micro pattern thereon.
Dependencies are inferred from the claim text.