Fusion proteins of Mycobacterium tuberculosis

An isolated fusion protein comprising the MTB32A antigen and the MTB39 antigen from a Mycobacterium species of the tuberculosis complex, wherein said fusion protein is encoded by a nucleic acid that hybridizes under highly stringent conditions to a nucleic acid comprising the nucleotide sequence of SEQ ID NO:1 or the complement thereof, and wherein the MTB32A antigen has a mutation at amino acid position 183 as compared to wild type MTB32A, wherein the highly stringent conditions comprise incubation in 50% formamide, 5×SSC, and 1% SDS at 42° C., or incubation in 5×SSC and 1% SDS at 65° C. with wash in 0.2×SSC and 0.1% SDS at 65° C.

26-57. (canceled)

An isolated fusion protein comprising the MTB32A antigen, the MTB39 antigen, and the MTB85B antigen from a Mycobacterium species of the tuberculosis complex, wherein said fusion protein is encoded by a nucleic acid that hybridizes under highly stringent conditions to a nucleic acid comprising the nucleotide sequence of SEQ ID NO:3 or the complement thereof. and wherein the MTB32A antigen has a mutation at amino acid position 183 as compared to wild type MTB32A, wherein the highly stringent conditions comprise incubation in 50% formamide, 5×SSC, and 1% SDS at 42° C. or incubation in 5×SSC and 1% SDS at 65° C. with wash in 0.2×SSC and 0.1% SDS at 65° C.

67-84. (canceled)

A fusion protein comprising an amino acid sequence selected from the group consisting of MTB89F (SEQ ID NO:13), MTB83F (SEQ ID NO:14), MTB81F (SEQ ID NO:15), MTB114F (SEQ ID NO:16), MTB102tm2F (SEQ ID NO:17) and MTB103F (SEQ ID NO:18).