Large-Scale Production of Human Serum Butyrylcholinesterase as a Bioscavenger
AssigneeThe United States of America as represented by the Secretary of the Army
InventorsBhupendra Doctor, Ashima Saxena, Wei Sun, Chunyuan Luo, Prasanthi Tipparaju, Irwin Koplovitz, David Lenz, Michelle Ross
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Abstract
Disclosed herein are methods for the large-scale preparation of human butyrylcholinesterase (HuBChE) preparations from Cohn Fraction IV-4. As disclosed, the methods provide HuBChE preparations that are about 99% or more pure with recovery yields of about 60%. Also disclosed are the pharmacokinetics, safety and toxicity, stability and efficacy of the HuBChE preparations.
Claims19
A method for obtaining a human butyrylcholinesterase preparation from a Cohn Fraction IV-4 paste of human origin said human butyrylcholinesterase preparation comprises 41.4 to 53.7% of the butyrylcholinesterase activity present in the Cohn Fraction IV-4 paste and has a purity of about 99% or more and a specific activity of about 700 U/mg, which comprises
- subjecting about 80 or more kilograms of the Cohn Fraction IV-4 paste to affinity chromatography only once and followed by subjecting the eluent to anion exchange chromatography only once.
Dependencies are inferred from the claim text.