Mammalian alpha-kinase proteins, nucleic acids and diagnostic and therapeutic uses thereof
AssigneeUniversity of Medicine and Dentistry of New Jersey
InventorAlexey Ryazanov
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Abstract
The present invention provides novel mammalian alpha-kinase proteins: melanoma alpha-kinase (MK), heart alpha-kinase (HK), kidney alpha-kinase (KK), skeletal muscle alpha-kinase (SK), and lymphocyte alpha-kinase (LK). In particular, a novel kinase type is herein provided, characterized by the presence of an alpha-kinase catalytic domain and an ion channel domain. Isolated nucleic acids of the alpha-kinases MK, HK, KK, SK and LK are provided. Methods for making the novel alpha-kinases, cells that express the alpha-kinases and methods for treating an animal in need of either increased or decreased activity of the alpha- kinases are provided.
Claims18
An isolated nucleic acid encoding mammalian melanoma alpha kinase, wherein the nucleic acid is selected from the group consisting of:
- a. the DNA sequence of SEQ ID NO:
- 28;
- b. the DNA sequence of SEQ ID NO:
- 26;
- c. DNA sequences that hybridize to the sequence of subparts (a) or (b) under standard hybridization conditions;
- and
- d. DNA sequences capable of encoding the amino acid sequence encoded by the DNA sequences of subparts (a), (b) or (c).
(canceled)
(canceled)
(canceled)
An isolated nucleic acid encoding mammalian kidney alpha kinase, wherein the nucleic acid is selected from the group consisting of:
- a. the DNA sequence of SEQ ID NO:
- 30;
- b. the DNA sequence of SEQ ID NO:
- 32;
- c. DNA sequences that hybridize to the sequence of subparts (a) or (b) under standard hybridization conditions;
- and
- d. DNA sequences capable of encoding the amino acid sequence encoded by the DNA sequences of subparts (a), (b) or (c).
An isolated nucleic acid encoding mammalian skeletal muscle alpha kinase, wherein the nucleic acid is selected from the group consisting of:
- a. the DNA sequence of SEQ ID NO:
- 38;
- b. DNA sequences that hybridize to the sequence of subpart (a) under standard hybridization conditions;
- and
- c. DNA sequences capable of encoding the amino acid sequence encoded by the DNA sequences of (a) or (b).
An isolated nucleic acid encoding mammalian lymphocyte alpha kinase, wherein the nucleic acid is selected from the group consisting of:
- a. the DNA sequence of SEQ ID NO:
- 40;
- b. DNA sequences that hybridize to the sequence of subpart (a) under standard hybridization conditions;
- and
- c. DNA sequences capable of encoding the amino acid sequence encoded by the DNA sequences of (a) or (b).
A unicellular host transformed with a recombinant DNA molecule comprising a DNA sequence or degenerate variant thereof, which encodes an alpha kinase, or a fragment thereof, selected from the group consisting of:
- a. the DNA sequence of (SEQ ID NO:
- 26);
- b. the DNA sequence of (SEQ ID NO:
- 28);
- c. the DNA sequence of (SEQ ID NO:
- 30);
- d. the DNA sequence of (SEQ ID NO:
- 32);
- e. the DNA sequence of (SEQ ID NO:
- 38);
- f. the DNA sequence of (SEQ ID NO:
- 40);
- g. DNA sequences that hybridize to any of the foregoing DNA sequences under standard hybridization conditions;
- and
- h. DNA sequences that code on expression for an amino acid sequence encoded by any of the foregoing DNA sequences;
- wherein said DNA sequence is operatively linked to an expression control sequence.
-48. (canceled)
Dependencies are inferred from the claim text.