Ex vivo and in vivo expression of the thrombomodulin gene for the treatment of cardiovascular and peripheral vascular diseases
AssigneeBiovec, LLC
InventorsLakshman Seghal, Jonathan Wong
Life of this patent
Estimating the term…
Abstract
The present invention relates to methods and compositions for treatment of cardiovascular and peripheral vascular diseases using ex vivo and in vivo gene delivery technologies. One aspect of the present invention relates to a method for treating a vascular disease by introducing a DNA sequence encoding a TM protein or its variant into a segment of a blood vessel in vivo using a gutless adenovirus vector. Another aspect of the present invention is to provide a method to deliver a gutless adenovirus vector carrying a DNA sequence encoding a TM protein or its variant using a stent.
Claims18
A method for treating a vascular disease in a mammal, said method comprising the steps of:
- evacuating a clot from a blood vessel in said mammal;
- isolating a segment of the blood vessel around the evacuation site;
- and
- infecting the segment of blood vessel in vivo using a gutless adenoviral vector comprising a polynucleotide encoding a thrombomodulin protein or its variant;
- wherein the thrombomodulin protein or its variant is expressed in a amount sufficient to reduce re-occlusion or intimal hyperplasia in the infected blood vessel, and wherein said gutless adenoviral vector comprises the nucleotide sequence of SEQ ID NO:13 or SEQ ID NO:15.
A method for treating a vascular disease in a mammal comprising
- administering a therapeutically effective amount of a gutless adenovirus vector into a segment of a blood vessel using a stent,
- wherein said gutless adenovirus vector comprises the nucleotide sequence of SEQ ID NO:
- 13 or SEQ ID NO:
- 15, and is capable of expressing a thrombomodulin protein or a variant of the thrombomodulin protein.
A method for treating a vascular disease in a mammal, said method comprising the steps of:
- administering intravenously an effective amount of a gutless adenoviral vector comprising a polynucleotide encoding a thrombomodulin protein or its variant,
- wherein said gutless adenoviral vector comprises the nucleotide sequence of SEQ ID NO:13 or SEQ ID NO:15.
Dependencies are inferred from the claim text.