Chimaeric vector system
AssigneeCambridge Enterprise Limited
InventorsAndrew Lever, Padraig Strappe
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Abstract
This invention relates to a process for producing a Simian Immunodeficiency Virus (SIV) encoding a heterologous gene, which process comprises infecting a host cell with a first vector which is capable of producing SIV capsid and a second vector comprising a Human Immunodeficiency Virus type 2 (HIV-2) packaging signal sufficient to package the second vector in the SIV capsid and a heterologous gene capable of being expressed by the vector; and culturing the host cell.
Claims46
A process for producing a chimaeric viral vector comprising;
- culturing a host cell which comprises one or more Simian Immunodeficiency Virus (SIV) nucleic acid sequences capable of producing an SIV capsid and which further comprises a vector comprising a Human Immunodeficiency Virus type 2 (HIV-2) packaging signal and a heterologous nucleic acid sequence;
- said vector being packaged in the SIV capsid to produce a chimaeric virus comprising the heterologous nucleic acid sequence.
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A process for producing a Simian Immunodeficiency Virus (SIV) encoding a heterologous gene, which process comprises:
- infecting a host cell with a first vector which is capable of producing SIV capsid and a second vector comprising a Human Immunodeficiency Virus type 2 (HIV-2) packaging signal sufficient to package the vector in the SIV capsid and a heterologous gene capable of being expressed by the vector;
- and
- culturing the host cell.
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A process for making a producer cell for the generation of chaemeric virus comprising:
- infecting a host cell which comprises one or more Simian Immunodeficiency Virus (SIV) nucleic acid sequences capable of producing an SIV capsid, with a vector comprising a Human Immunodeficiency Virus type 2 (HIV-2) packaging signal and a heterologous nucleic acid sequence.
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A vector system comprising a first vector which is capable of producing SIV capsid and a second vector comprising a Human Immunodeficiency Virus type 2 (HIV-2) packaging signal sufficient to package the vector in the SIV capsid and a cloning site suitable for insertion of a heterologous gene capable of being expressed by the vector.
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Dependencies are inferred from the claim text.