Methods and compositions for diagnosis and immunotherapy of pollen allergy
AssigneeThe University of Chicago
InventorsDaphne Preuss, Ravishankar Palanivelu, Katinka Vigh, Matthew Cummings, Mohamed Bashir
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Abstract
A diagnostic pollen array includes allergens extracted from pollen coat material and pollen cytoplasm. Diagnostic pollen arrays are useful to diagnose allergy in individuals, identify novel allergens, identify genetic loci responsible for allergy in hosts, and to develop personalized treatment plans for allergy.
Claims45
A pollen array comprising a pollen surface allergen.
A method of making a pollen array, the method comprising:
- (a) obtaining pollen from an allergenic plant species;
- (b) preparing a pollen cell surface extract from the pollen;
- (c) obtaining at least one pollen allergen from the pollen extract;
- and
- (d) applying the pollen allergen to a solid support in an orderly fashion in the array.
A method of preparing a pollen surface allergen extract, the method comprising:
- (a) obtaining an unwashed or untreated pollen from an allergenic plant species;
- (b) exposing the unwashed or untreated pollen to an organic solvent to separate the surface pollen;
- and
- (c) obtaining the surface pollen allergen extract.
A method of preparing a pollen extract comprising pollen surface allergens and pollen cytoplasmic allergens, the method comprising:
- (a) obtaining pollen from an allergenic plant species;
- (b) washing the pollen with an organic solvent to separate the surface pollen allergens;
- (c) lysing the washed pollen in an aqueous solution to obtain the cytoplasmic allergens;
- (d) obtaining the pollen extract comprising the pollen surface allergens and pollen cytoplasmic allergens.
An isolated allergenic pollen peptide from Bermuda grass comprising an amino acid sequence WVIENGGITTLADYPYR.
Dependencies are inferred from the claim text.