Electrical measurement of cell invasion
OwnerIvar Giaever, Charles R. Keeseinventors
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Abstract
The electrical measurement of invasive cell movement is provided. A gel layer is added to a well including a substrate having an electrode thereon. A medium is added over the gel layer. One of the medium and the gel layer can include cells and the other of the medium and the gel layer can comprise a compound to be evaluated for its influence on cell migration. An electrical signal is applied to the electrode and a property (e.g., impedance) of the electrical signal is measured. By analyzing the property, it can be determined whether and to what extent cells are in contact with the electrode. An influence of the compound on cell migration can be correlated with the property.
Claims22
A method of electrically measuring invasive cell movement, the method comprising:
- adding cells over a first gel layer in a well, the well including a substrate having an electrode;
- applying an electrical signal to the electrode;
- and
- measuring at least one property of the electrical signal.
A method of electrically measuring invasive cell movement, the method comprising:
- adding a medium over a first gel layer and cells in a well, the well including a substrate having an electrode;
- applying an electrical signal to the electrode;
- and
- measuring at least one property of the electrical signal.
A method of electrically evaluating an influence of a compound on cell migration, the method comprising:
- adding a medium over a gel layer in a first well, the first well including a substrate having an electrode, one of the medium and the gel layer including cells and the other of the medium and the gel layer including the compound;
- applying an electrical signal to the electrode;
- and
- measuring at least one property of the electrical signal.
Dependencies are inferred from the claim text.