Method of Preparing a Composition Using Argentation Chromatography

A method of preparing an argentized alumina adsorbent, comprising:

• (a) providing an argentized alumina adsorbent having:
• i. an initial selectivity (B1);
• and,
• ii. an initial Hunter L color value (IHC);
• (b) subjecting said argentized alumina to a conditioning process for at least about 24 hours whereby a conditioned argentized alumina absorbent is prepared having:
• i. a second selectivity (B2);
• and,
• ii. a second Hunter L color value (HC-2);
• wherein, B1 is a value between about the value of B2 and about 1.000, and HC-2 is at least about 10% lower than IHC.

A method of preparing an enriched composition, wherein said method is a simulated moving bed chromatographic process comprising:

• (a) combining a first composition comprising:
• i. at least one compound containing at least one saturated C12-24 carbon chain and
• ii. at least one compound containing at least one unsaturated C12-24 carbon chain
• with an argentized adsorbent selected from the group consisting of a cationic resin and a conditioned alumina;
• wherein said adsorbent is contained on chromatographic beds, columns or parts thereof arranged in a simulated moving bed chromatographic array;
• (b) contacting said combined first composition and said adsorbent with one or more solvent(s) simultaneously or in sequence;
• and
• (c) eluting a second composition that is enriched relative to said first composition in said at least one compound containing at least one unsaturated C12-24 carbon chain;
• wherein a second composition enriched in said at least one compound containing an unsaturated C12-24 carbon chain is prepared.

A method of preparing an enriched composition, wherein said method is a simulated moving bed chromatographic process comprising:

• (a) combining a first composition comprising:
• i. at least one compound containing at least one monounsaturated C12-24 carbon chain and
• ii. at least one compound containing at least one polyunsaturated C12-24 carbon chain
• with an argentized adsorbent selected from the group consisting of a cationic resin and a conditioned alumina;
• wherein said adsorbent is contained on chromatographic beds, columns or parts thereof arranged in a simulated moving bed chromatographic array;
• (b) contacting said combined first composition and said adsorbent with one or more solvent(s) simultaneously or in sequence;
• and
• (c) eluting a second composition that is enriched relative to said first composition in said at least one compound containing at least one polyunsaturated C12-24 carbon chain;
• wherein a second composition enriched in said at least one compound containing a polyunsaturated C12-24 carbon chain is prepared.