Method and apparatus for packing chromatography column
AssigneeAgilent Technologies, Inc.
InventorsBernd-Walter Hoffmann, Brian Bidlingmeyer, Alan Broske
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Abstract
An apparatus for packing a chromatography column with a granular packing medium is disclosed. The apparatus includes a chamber adapted to hold a mixture of the packing medium and a liquid. The chamber is in fluid communication with a pump and the column. A vibrator is positioned so as to agitate the mixture within the chamber. The invention also includes a method of packing the chromatography column by forming a mixture comprising the packing medium and the liquid, positioning the mixture within the chamber, agitating the mixture, and pumping liquid into the chamber thereby forcing the mixture through the column.
Claims17
An apparatus for packing a chromatography column with a granular packing medium using a transport liquid, said apparatus comprising:
- a chamber adapted to hold a mixture of said packing medium and said liquid, said chamber being in fluid communication with said column;
- a vibrator positioned so as to agitate said mixture within said chamber;
- and
- a pump in fluid communication with said chamber to pump said liquid from said chamber into said column.
An apparatus for packing a chromatography column with a granular packing medium by passing a liquid carrying said packing medium through said column, said apparatus comprising:
- a pump for pumping said liquid;
- a chamber adapted to hold a mixture comprising said packing medium and said liquid;
- a first conduit providing fluid communication between said pump and said chamber;
- a second conduit providing fluid communication between said chamber and said column;
- a vibrator positioned so as to agitate said mixture within said chamber;
- and wherein
- said liquid is pumped into said chamber, said mixture within said chamber being thereby forced through said column, said vibrator agitating said mixture within said chamber to provide a substantially uniform distribution of said packing medium throughout said liquid, said packing medium being retained within said column.
A method of packing a chromatography column with a granular packing medium carried by a liquid, said method comprising:
- forming a mixture comprising said packing medium and said liquid;
- positioning said mixture within a chamber in fluid communication with said column;
- agitating said mixture within said chamber;
- pumping said liquid into said chamber thereby forcing said mixture through said column, said packing medium being retained within said column.
Dependencies are inferred from the claim text.