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AssigneeGL Sciences Incorporated
InventorsAbudogupur Abudokirim, Masayoshi Ohira, Kensuke Okusa, Nobuo Seto, Masahiro Furuno
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A high-purity fragment is obtained by a simple mechanism and method for separating and purifying a nucleic acid, particularly fragment DNA, extremely efficiently and with a high reproducibility, wherein elution with a high-concentration salt is not performed and necessity of elution and purification is eliminated. This mechanism is a mechanism for purifying a nucleic acid, particularly fragment DNA using a monolith structure formed with glass or silica, specifically, an integral porous body having an open structure with pores that communicate the upper end with the lower end, wherein through-pores corresponding to nucleic acid sizes of 35 bp (mer) to 100 Kbp (mer) are provided.
An apparatus for separating and purifying nucleic acids comprising an integral monolith structure, wherein through-pores (macro-pores) continuously extending from one end of the monolith structure to the other end and corresponding to the sizes of nucleic acids are provided and configured so that nucleic acids corresponding to the through-pores (macro-pores) can be retained respectively by allowing a solution containing nucleic acids to be separated to pass therethrough.
A method for separating and purifying nucleic acids comprising a step of using an integral monolith structure, wherein through-pores (macro-pores) continuously extending from one end of the monolith structure to the other end and corresponding to the sizes of nucleic acids are provided and configured so that nucleic acids corresponding to the through-pores (macro-pores) can be retained respectively by allowing a solution containing nucleic acids to be separated to pass therethrough.
Dependencies are inferred from the claim text.