Back to results
US 2007/0179289 A1Published application
Compositions and methods for the use of FMOC derivatives in DNA/RNA synthesis
AssigneeSyngen, Inc.
InventorGabriel Alvarado
Life of this patent
Estimating the term…
Abstract
Methods of nucleic acid preparation are described, including preparation of mRNA, using FMOC derivatives to synthesize oligonucleotides in addition to applying FMOC protocols to various therapeutic and diagnostic methods. In some embodiments a single stranded oligonucleotide is synthesized bound to a polymer support (such as optic fiber glass filters) using said FMOC derivatives.
Claims4
A method for synthesizing and purifying a phosphotriester oligonucleotide immobilized on a solid support complex, comprising:
- a) providing i) a polymer support, and ii) four pools of nucleoside 3′-monomers in solution, said monomers having a nucleoside base and a phosphoramidate group, wherein at least one of said monomers contains 9-fluorenylmethoxycarbonyl as an amino protecting group on the monomer base, and said monomers have an alkyl residue on the phosphate of the phosphoramidite group;
- b) sequentially contacting said polymer support with a solution of monomers from one or more of said four pools under conditions such that a sequence of monomers is immobilized on said polymer support to generate an oligonucleotide/polymer support complex, wherein said complex comprises at least one monomer containing 9-fluorenylmethoxycarbonyl base protecting group and wherein said solution, after said contacting, comprises unreacted material;
- c) treating said complex under conditions such that said unreacted material is substantially removed, thereby creating a purified oligonucleotide/polymer support complex, and
- d) treating said purified oligonucleotide/polymer support complex under conditions whereby said 9-fluorenylmethoxycarbonyl, but not the phosphoramidate alkyl residue, is released.
1 more element
Dependencies are inferred from the claim text.