Extraction process for a pharmaceutical product
OwnerBhanu Manickavasagaminventor
Life of this patent
Estimating the term…
Abstract
A process for isolating a soluble, native collagen from a marine invertebrate, comprising the steps of: 1) treating a collagen-containing portion of the marine invertebrate with a weak acid solution in order to solubilise native collagen fibrils; 2) centrifuging the resultant slurry to remove tissue particulates; 3) adjusting the pH of the supernatant in order to precipitate collagen by addition of a base; 4) collecting the precipitated collagen; 5) resuspending the precipitated collagen; and performing buffer exchange against water using an 15 ultrafiltration membrane.
Claims37
A process for isolating a soluble, native collagen from a marine invertebrate, comprising the steps of:
- 1) treating a collagen-containing portion of the marine invertebrate with a weak acid solution in order to solubilise native collagen fibrils;
- 2) centrifuging the resultant slurry to remove tissue particulates;
- 3) adjusting the pH of the supernatant in order to precipitate collagen by addition of a base;
- 4) collecting the precipitated collagen;
- 5) resuspending the precipitated collagen;
- and
- 6) performing buffer exchange against water using an ultrafiltration membrane.
A method of preparing a dried native collagen film suitable for use as a cell culture medium, comprising the steps of:
- (1) providing a solution of native collagen derived from abalone;
- (2) applying the solution to a surface so as to coat the surface with a film of native collagen derived from abalone;
- and
- (3) drying the film to form a dried collagen film on the surface.
A cell culture vessel coated at least in part with native collagen derived from abalone.
A cell culture medium comprising native collagen derived from abalone.
A kit for cell culture comprising:
- (a) native collagen derived from abalone;
- and
- (b) a weak acid solution for admixture with the native collagen derived from abalone to enable preparation of a solution of native abalone collagen.
Dependencies are inferred from the claim text.