BoNT/A Peptides and Methods of Predicting and Reducing Immunoresistance to Botulinum Toxin Therapy

An isolated BoNT/A peptide of SEQ ID NO:

• 1 consisting essentially of an amino acid sequence selected from the group consisting of amino acids 491-509 of SEQ ID NO:
• 1, amino acids 519-537 of SEQ ID NO:
• 1, amino acids 533-551 of SEQ ID NO:
• 1, amino acids 547-565 of SEQ ID NO:
• 1, amino acids 589-607 of SEQ ID NO:
• 1, amino acids 631-649 of SEQ ID NO:
• 1, amino acids 659-677 of SEQ ID NO:
• 1, amino acids 673-691 of SEQ ID NO:
• 1, 715-733 of SEQ ID NO:
• 1, amino acids 743-761 of SEQ ID NO:
• 1, amino acids 771-789 of SEQ ID NO:
• 1, amino acids 785-803 of SEQ ID NO:
• 1, amino acids 813-831 of SEQ ID NO:
• 1, amino acids 827-845 of SEQ ID NO:
• 1 and a conservative BoNT/A amino acid sequence variant thereof;
• wherein said conservative BoNT/A amino acid sequence variant comprises 1-4 conservative amino acid substitutions to amino acids 491-509 of SEQ ID NO:
• 1, 1-4 conservative amino acid substitutions to amino acids 519-537 of SEQ ID NO:
• 1, 1-4 conservative amino acid substitutions to amino acids 533-551 of SEQ ID NO:
• 1, 1-4 conservative amino acid substitutions to amino acids 547-565 of SEQ ID NO:
• 1, 1-4 conservative amino acid substitutions to amino acids 589-607 of SEQ ID NO:
• 1, 1-4 conservative amino acid substitutions to amino acids 631-649 of SEQ ID NO:
• 1, 1-4 conservative amino acid substitutions to amino acids 659-677 of SEQ ID NO:
• 1, 1-4 conservative amino acid substitutions to amino acids 673-691 of SEQ ID NO:
• 1, 1-4 conservative amino acid substitutions to amino acids 715-733 of SEQ ID NO:
• 1, 1-4 conservative amino acid substitutions to amino acids 743-761 of SEQ ID NO:
• 1, 1-4 conservative amino acid substitutions to amino acids 771-789 of SEQ ID NO:
• 1, 1-4 conservative amino acid substitutions to amino acids 785-803 of SEQ ID NO:
• 1, 1-4 conservative amino acid substitutions to amino acids 813-831 or 1-4 conservative amino acid substitutions to amino acids 827-845 of SEQ ID NO:
• 1.

An isolated BoNT/A peptide of SEQ ID NO:

• 1 having a length of at most 30 amino acids, said BoNT/A peptide comprising an amino acid sequence selected from the group consisting of amino acids 554-564 of SEQ ID NO:
• 1, amino acids 593-602 of SEQ ID NO:
• 1, amino acids 666-676 of SEQ ID NO:
• 1, amino acids 748-757 of SEQ ID NO:
• 1, amino acids 785-794 of SEQ ID NO:
• 1 and a conservative BoNT/A amino acid sequence variant thereof;
• wherein said conservative BoNT/A amino acid sequence variant comprises 1-4 conservative amino acid substitutions to amino acids 554-564 of SEQ ID NO:
• 1, 1-4 conservative amino acid substitutions to amino acids 593-602 of SEQ ID NO:
• 1, 1-4 conservative amino acid substitutions to amino acids 666-676 of SEQ ID NO:
• 1, 1-4 conservative amino acid substitutions to amino acids 748-757 of SEQ ID NO:
• 1, 1-4 conservative amino acid substitutions to amino acids 785-794 of SEQ ID NO:
• 1.

An isolated BoNT/A peptide of SEQ ID NO:

• 1 consisting essentially of an amino acid sequence selected from the group consisting of amino acids 554-564 of SEQ ID NO:
• 1, amino acids 593-602 of SEQ ID NO:
• 1, amino acids 666-676 of SEQ ID NO:
• 1, amino acids 748-757 of SEQ ID NO:
• 1, amino acids 785-794 of SEQ ID NO:
• 1, and a conservative BoNT/A amino acid sequence variant thereof;
• wherein said conservative BoNT/A amino acid sequence variant comprises 1-4 conservative amino acid substitutions to amino acids 554-564 of SEQ ID NO:
• 1, 1-4 conservative amino acid substitutions to amino acids 593-602 of SEQ ID NO:
• 1, 1-4 conservative amino acid substitutions to amino acids 666-676 of SEQ ID NO:
• 1, 1-4 conservative amino acid substitutions to amino acids 748-757 of SEQ ID NO:
• 1, 1-4 conservative amino acid substitutions to amino acids 785-794 of SEQ ID NO:
• 1.