Highly purified proteinase k
AssigneeRoche Diagnostics Operations, Inc.
InventorsHelmut Schoen, Guenter Guertler, Bernhard Rexer, Johann-Peter Thalhofer
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Abstract
A method for producing solid proteinase K which is insoluble in water and has high purity, the method comprising the steps of adding to an aqueous solution of proteinase K ammonium sulfate in an amount of 0.1 to 0.2 M per 5 minutes up to a final amount of 1.5 to 2 M after 1 hour, thereby precipitating the proteinase K as a solid, and isolating the solid proteinase K.
Claims12
A method for producing solid proteinase K which is insoluble in water, the method comprising the steps of:
- adding to an aqueous solution of proteinase K ammonium sulfate in an amount of 0.1 to 0.2 M per 5 minutes up to final amount of 1.5 to 2 M after 1 hour, thereby precipitating the proteinase K as a solid, and
- isolating the solid proteinase K.
A method for the purification of proteinase K comprising the steps of:
- adding to an aqueous solution of proteinase K ammonium sulfate in an amount of 0.1 to 0.2 M per 5 minutes up to a final amount of 1.5 to 2 M after 1 hour, thereby precipitating the proteinase K as a solid,
- isolating the solid proteinase K,
- resuspending the isolated solid proteinase K in water or an aqueous buffer solution, and
- isolating the resuspended proteinase K.
A method for producing solubilized proteinase K comprising the step of:
- adding to an aqueous solution of proteinase K ammonium sulfate in an amount of 0.1 to 0.2 M per 5 minutes up to a final amount of 1.5 to 2 M after 1 hour, thereby precipitating the proteinase K as a solid,
- isolating the solid proteinase K, and
- adding to the isolated proteinase K an aqueous solution of glycerol at a concentration of 40% to 60% (w/v), thereby solubilizing the proteinase K.
Dependencies are inferred from the claim text.