Back to results
US 2007/0178602 A1Published application
Method to be used in fluoresence microscopy
AssigneeCarl Zeiss MicroImaging GmbH
InventorsRalf Wolleschensky, Bernhard Zimmermann, Richard Ankerhold
Life of this patent
Estimating the term…
Abstract
A method using fluorescence microscopy for image evaluation using a laser scanning microscope in which an at least partially spectrally resolved detection of the fluorescence spectrum occurs. Reference spectra are used for spectral demixing. Temporally and/or spectrally variable dyes and/or dye combinations are employed for recording of the reference spectra. Finally, the recorded reference spectra are inspected for image evaluation.
Claims13
1-12. (canceled)
A method using fluorescence microscopy for image evaluation, the method comprising the steps of:
- using a laser scanning microscope in which an at least partially spectrally resolved detection of the fluorescence spectrum occurs;
- using reference spectra for spectral demixing;
- employing temporally and/or spectrally variable dyes and/or dye combinations for recording of the reference spectra;
- and
- demixing the recorded reference spectra for image evaluation.
2 more elements
Dependencies are inferred from the claim text.