Novel dominant selection marker for the transformation of fungi

Abstract

The invention relates to a new expression vector for the transformation of eukaryotic cells, in particular fungal cells, or eukaryotic cell organelles as well as to a method for the transformation of eukaryotes, in particular fungi, or eukaryotic cell organelles employing these expression vector. The expression vector comprises at least one acc gene encoding at least one subunit of a MS-type acetyl-CoA carboxylase (MS-ACC), placed under the control of eukaryotic expression signals, and is a suitable selection marker for the transformation of eukaryotic cells, in particular fungal cells, or eukaryotic cell organelles to resistance to an inhibitor of MF-type acetyl-CoA carboxylases. The method involves the application of one or several acc genes encoding one or several subunits of a MS-type acetyl-CoA carboxylase (MS-ACC), as selection marker for the transformation of eukaryotic cells, in particular fungal cells, or eukaryotic cell organelles to resistance to an inhibitor of MF-type acetyl-CoA carboxylases, whereby the respective gene or genes is/are part of said expression vector and is/are under the control of eukaryotic expression signals.

Dependencies are inferred from the claim text.