Particle Preparation for Direct-Delivery Transformation
AssigneePioneer Hi-Bred International, Inc.
InventorsMichael Miller, Gary Sandahl, David Peterson, Bruce Drummond, Mark Chamberlin, Benjamin Bowen, Grace St. Clair, William Gordon-Kamm
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Abstract
Methods of preparing microparticles for transformation of plant cells with compositions of interest, and compositions comprising the prepared particles and the associated compound(s) of interest are provided. Further provided are methods to deliver compositions of interest to plant cells for transient or stable incorporation in the genome, and any transformed plant cells, plants, and seeds produced thereby.
Claims36
A method to prepare microparticles for direct delivery of a composition of interest to plant cells comprising:
- a) providing microparticles suitable for direct-delivery into plant cells;
- b) providing the composition of interest;
- and,
- c) contacting the composition of interest with the microparticles in the presence of a compound to produce a microparticle having the composition of interest attached thereto, wherein the compound is selected from the group consisting of a cationic lipid solution, a liposome solution, a cationic polymer, a cationic protein, a cationic peptide, and a cationic polyamino acid.
A direct-delivery composition comprising microparticles, a composition of interest, and a compound selected from the group consisting of a cationic lipid solution, a liposome solution, a cationic polymer, a cationic protein, a cationic peptide, and a cationic polyamino acid.
A method to provide a composition of interest to plant cells comprising:
- a) providing microparticles suitable for direct delivery into plant cells;
- b) providing the composition of interest;
- c) contacting the composition of interest with the microparticles in the presence of a compound to produce microparticles having the composition of interest attached thereto, wherein the compound is selected from the group consisting of a cationic lipid solution, a liposome solution, a cationic polymer, a cationic protein, a cationic peptide, and a cationic polyamino acid;
- and,
- d) contacting the microparticles produced in step (c) with plant cells such that the microparticles deliver the composition of interest to the interior of the plant cells.
Dependencies are inferred from the claim text.