Methods and apparatuses for growing cells
AssigneeThe Ohio State University Research Foundation
InventorsShang-Tian Yang, Anil Ouyang
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Abstract
Methods of culturing stem cells including growing fibroblast cells on a three-dimensional scaffold, perfusing the fibroblast cells with a cell culture medium to form fibroblast cell-conditioned cell culture medium, and growing the stem cells on a three-dimensional scaffold perfused with the fibroblast cell-conditioned cell culture medium are presented. Multi-stage bioreactors for growing stem cells, comprising a first fibrous bed bioreactor in fluid communication with a second fibrous bed bioreactor are also presented.
Claims29
A method of culturing stem cells comprising:
- growing the stem cells in a first bioreactor comprising a three-dimensional scaffold;
- co-culturing in a second bioreactor feeder cells and delivering, either intermittently or continuously, media from the second bioreactor to the first bioreactor;
- and
- perfusing the stem cells with culture medium.
A multi-stage bioreactor for growing embryonic stem cells, comprising a first fibrous bed bioreactor in fluid communication with a second fibrous bed bioreactor, wherein the first fibrous bed bioreactor is adapted for growing embryonic stem cells.
Dependencies are inferred from the claim text.