Screening method and apparatus for identifying a reactive compound bonding with a target compound

A screening apparatus configured to identify a reactive compound bonding with a target compound, comprising:

• a first dimension passage configured to lead a sample injected into a sample injection port to a first dimension column using a first dimension mobile phase to separate the sample into components,
• a sample supply unit configured to inject the sample into said sample injection port,
• an isolation passage configured to isolate a portion of separated sample components containing reaction products resulting from bonding between the target and reactive compounds into a loop passage,
• a reactive compound concentration passage for leading an eluant into a trap column, said eluant dissociating said reactive compound from said reaction products as a concentration mobile phase, for selectively trapping said reactive compound while dissociating it from the reaction products isolated by the isolation of the sample;
• and
• a second dimension passage configured to lead the sample trapped in said trap column to a second dimension column using a second dimension mobile phase.