Mosaic infectious bursal disease virus vaccines
AssigneeID-Lelystad, Instituut voor Dierhouderij en Diergezondheid B.V.
InventorsHendrik Boot, Anna ter Huurne, Bernardus Peeters
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Abstract
The invention relates to Infectious Bursal Disease Virus (“IBDV”) and vaccines therefor. Provided are infectious recombinant Infectious Bursal Disease Virus (“rIBDV”) essentially incapable of growing in a cell that is not derived from a bursa cell, or an infectious rIBDV having retained at least part of the very virulent characteristics of a very virulent Infectious Bursal Disease Virus (“vvIBDV”).
Claims20
An infectious recombinant Infectious Bursal Disease Virus (rIBDV) essentially incapable of growing in a non-bursa cell or cell derived from a non-bursa cell.
An infectious rIBDV having retained at least part of the very virulent characteristics of a very virulent Infectious Bursal Disease Virus (vvIBDV).
(canceled)
A method for obtaining an infectious recombinant Infectious Bursal Disease Virus (rIBDV) having retained at least part of the very virulent characteristics of a very virulent Infectious Bursal Disease Virus (vvIBDV), said method comprising:
- transfecting at least one first cell with a nucleic acid comprising an IBDV genome at least partly derived from a vvIBDV;
- incubating said at least one first cell in a culture medium;
- recovering rIBDV from said at least one transfected first cell or said culture medium;
- and
- propagating said recovered rIBDV in at least one second cell permissive for said vvIBDV.
12-22. (canceled)
An infectious mosaic IBDV (mIBDV) comprising an rIBDV wherein at least one genome segment comprises nucleic acid derived from at least two different Birna virus isolates.
Dependencies are inferred from the claim text.