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US 2007/0178554 A1Published application
Orthogonal Aminoacyl Synthetase-tRNA Pairs for Incorporating Unnatural Amino Acids Into Proteins
OwnerNima Shiva, Mark Nowak +1inventors
InventorsNima Shiva, Mark Nowak, Niki Zacharias
Life of this patent
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Abstract
Methods of incorporating unnatural amino acids into proteins in eukaryotic cells using an orthogonal aminoacyl synthetase and an orthogonal tRNA derived from Lactococcus lactis.
Claims18
A translation system comprising:
- (a) translation components derived from a eukaryotic organism;
- and
- (b) an aminoacyl synthetase/tRNA pair derived from an organism selected from the group consisting of Lactococcus lactis, Gluconobacter oxydans and Rhodospirullum rubrum, wherein the aminoacyl synthetase and tRNA are orthogonal with respect to the translation components derived from the eukaryotic organism, and wherein the tRNA is aminoacylated with an unnatural amino acid by the aminoacyl synthetase with enhanced efficiency as compared to aminoacylation of the tRNA by the aminoacyl synthetase with a natural amino acid.
A vector comprising:
- (a) a first nucleic acid molecule comprising a first nucleic acid sequence that encodes an aminoacyl synthetase derived from Lactococcus lactis;
- and
- (b) a second nucleic acid molecule comprising a second nucleic acid sequence that encodes a tRNA derived from Lactococcus lactis that is aminoacylated with an unnatural amino acid by the aminoacyl synthetase derived from Lactococcus lactis, wherein the tRNA comprises an anticodon loop having a sequence that specifically binds a selector codon of an mRNA molecule.
Dependencies are inferred from the claim text.