Method of isolating a protein
AssigneeProteome Systems Intellectual Property Pty Ltd.
InventorsSusanne Pedersen, Robert Cole, Ron Weinberger, Andrew Sloane
Life of this patent
Estimating the term…
Abstract
The present invention provides a method for isolating and/or identifying an immunogenic protein from a protein complex comprising an immunoglobulin or a mixture thereof or an immunoglobulin-containing fraction.
Claims54
A method for identifying an immunogenic protein or fragment thereof capable of eliciting an immune response, said method comprising obtaining a protein complex comprising an immunoglobulin or mixtures thereof or an immunoglobulin-containing fraction from a subject or a cell, tissue or organ thereof and identifying a protein or fragment thereof bound to the immunoglobulin by virtue of an antigen antibody interaction, thereby identifying an immunogenic protein or fragment thereof capable of eliciting an immune response.
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44-64. (canceled)
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69-94. (canceled)
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124-125. (canceled)
The method according to claim 125 wherein the subject suffers from an infection caused by Pseudomonas aeruginosa.
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A method comprising:
- (a) obtaining a protein complex comprising an immunoglobulin or mixtures thereof or an immunoglobulin-containing fraction from a subject that has raised an immune response against an immunogenic protein or fragment thereof or a cell, tissue or organ thereof by a method comprising contacting a sample from the subject with one or more compounds capable of binding an immunoglobulin for a time and under conditions sufficient for binding to occur and isolating the one or more compounds;
- (b) linking immunoglobulin in the protein complex or immunoglobulin-containing fraction to the one or more compounds;
- (c) separating an immunogenic protein or fragment thereof from the linked immunoglobulin;
- (d) contacting a sample comprising the immunogenic protein or fragment thereof with the linked immunoglobulin;
- (e) optionally, separating the immunogenic protein or fragment thereof from the linked immunoglobulin;
- (f) repeating (d) and (e) one or more times;
- and
- (g) identifying a protein or fragment thereof separated from the immunoglobulin, thereby identifying an immunogenic protein or fragment thereof.
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Dependencies are inferred from the claim text.