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US 2007/0178538 A1Published application
Methods for measuring glycan levels of proteins
AssigneeVan Andel Research Institute
InventorBrian Haab
Life of this patent
Estimating the term…
Abstract
The invention includes a high-through-put method of screening for a biomarker indicative of a disease, including the identification of a glycan alteration in a protein of a diseased patient. Further, the invention includes methods utilizing a microarray to measure the glycan level of a protein.
Claims24
A high-throughput method of screening for a biomarker indicative of a disease, comprising the following steps:
- providing serum samples from diseased patients who are afflicted with the disease;
- providing serum samples from healthy patients who are not afflicted with the disease;
- determining whether a glycan alteration in a protein in the diseased patient serum samples is present at a different level than in the protein in the healthy patient serum samples;
- and
- if the diseased patient serum samples have said different level, identifying the glycan alteration in the protein as a biomarker indicative of a disease.
2 more elements
A method of measuring the glycan level of a protein comprising the following steps:
- providing a biological sample from a patient;
- providing a group of antibodies that bind specifically to serum proteins in the sample;
- spotting the group of antibodies at particular addresses on a microarray;
- labeling the serum proteins in the sample with a first tag;
- incubating the sample on the microarray such that the serum proteins in the sample are bound to the antibodies on the microarray;
- washing off unbound serum proteins from the microarray;
- providing a glycan binding protein labeled with a second tag;
- incubating the labeled glycan binding protein on the microarray such that the labeled glycan binding protein binds to a glycan on the serum proteins that are bound to the antibodies on the microarray;
- washing off the unbound labeled glycan binding protein;
- and
- detecting the levels of the first tag and the second tag at the particular addresses of the microarray.
8 more elements
A method of measuring the glycan level of a protein, comprising the following steps:
- providing a biological sample from a patient;
- providing a group of antibodies that bind specifically to serum proteins in the sample;
- spotting the group of antibodies at particular addresses on a microarray;
- incubating the sample on the microarray such that serum proteins in the sample are bound to the antibodies on the microarray;
- washing off unbound serum proteins from the microarray;
- providing a first glycan binding protein tagged with a first tag and a second glycan binding protein tagged with a second tag;
- incubating said first tagged glycan binding protein and said second tagged glycan binding protein on the microarray;
- washing off unbound first tagged glycan binding protein and unbound second tagged glycan binding protein;
- and
- detecting the levels of the first and second tags at particular addresses on the microarray.
7 more elements
Dependencies are inferred from the claim text.