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US 2007/0178534 A1Published application
Substrates, devices, and methods for cellular assays
AssigneePlatypus Technologies, LLC
InventorsChristopher Murphy, Barbara Israel, Nicholas Abbot
Life of this patent
Estimating the term…
Abstract
The present invention relates to the field of molecular diagnostics, and in particular to diagnostics based on a liquid crystal assay format. In particular, the present invention provided improved substrates and methods of using liquid crystal assays for quantitating the amount of an analyte in a sample. The present invention also provides materials and methods for detecting non-specific binding of an analyte to a substrate by using a liquid crystal assay format.
Claims17
1-97. (canceled)
A method for assaying cell movement comprising:
- a) providing:
- i) a multiwell plate comprising masks for wells in said multiwell plate, wherein said masks provide a masked portion and unmasked portion in said wells;
- ii) a cell seeding device configured for use with said multiwell plate;
- and
- iii) cells
- b) inserting said cell seeding device into at least one well of said multiwell plate, wherein said cell seeding device defines a predetermined area in said at least one well;
- c) contacting said at least one well in said multiwell plate with said cells via said cell seeding device under conditions such that said cells are seeded in said predetermined area;
- d) removing said cell seeding device;
- e) culturing said cells;
- f) assaying said cells for movement away from said predetermined area, wherein said cells are assayed in said unmasked portion in said wells.
7 more elements
A cell assay system comprising:
- a) a multiwell plate comprising masks for wells in said multiwell plate, wherein said masks provide a masked portion and unmasked portion in said wells, wherein said wells have a bottom surface;
- b) a cell seeding device insertable into at least one well of said multiwell plate, wherein said cell seeding device defines a predetermined area on said bottom surface of said at least one well, so that when cells are applied to said at least one well said cells are seeded in said predetermined area.
A cell assay kit comprising:
- a) a multiwell plate comprising masks for wells in said multiwell plate, wherein said masks provide a masked portion and unmasked portion in said wells, wherein said wells have a bottom surface;
- b) a cell seeding device insertable into at least one well of said multiwell plate, wherein said cell seeding device defines a predetermined area on said bottom surface of said at least one well, so that when cells are applied to said at least one well said cells are seeded in said predetermined area.
Dependencies are inferred from the claim text.