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AssigneeThe Regents of the University of California
InventorsLewis Williams, Jaime Escobedo
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DNA sequences encoding human platelet-derived growth factor receptors (hPDGF-R), and expression constructs comprising sequences which encode a receptor that can be secreted or incorporated into the membrane of a mammalian cell. Peptide fragments with functions equivalent to the wild-type receptor, conferring a PDGF-sensitive mitogenic response on cells lacking the receptor are provided. The constructs can be used for enhancing PDGF response of cells, determining the regions involved in transducing the signal in response to PDGF binding, providing mutated analogs and evaluating drugs for their physiologic activity. Soluble fragments comprising PDGF receptor sequences are also provided, including important intracellular kinase insert sequences which interact with intracellular proteins.
A purified and isolated recombinant nucleic acid of less than about 50 kbp comprising at least about 24 contiguous nucleotides which encode a human platelet-derived growth factor receptor (hPDGF-R) polypeptide segment.
A substantially pure hPDGF-R polypeptide fragment of at least about twenty amino acids having platelet-derived growth factor (PDGF) receptor binding activity or tyrosine kinase activity.
A composition comprising an unglycosylated hPDGF-R fragment.
A composition comprising a hPDGF-R fragment, which exhibits a glycosylation pattern which is non-fungal and non-human.
An isolated hPDGF-R polypeptide of less than about 200 amino acids comprising a receptor kinase insert region.
Dependencies are inferred from the claim text.