Methods for determining and lowering caffeine concentration in fluids
AssigneeWashington University
InventorsJack Ladenson, Ruth Ladenson, Yvonne Landt, Dan Crimmins
Life of this patent
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Abstract
Single-chain, camelized heavy chain antibodies immunospecific for caffeine and stable at high temperatures are useful for analysis and recovery of caffeine in or from fluids. A device that provides a single-step lateral flow assay for caffeine and a useful peptide spacer are also described.
Claims33
A moiety that has the property of binding to caffeine at 70° C.
A lateral flow device having three reactive zones sequentially arranged in a same or substantially same plane wherein the first zone comprises a mobile, labeled caffeine-binding moiety, the second zone comprises a stationary caffeine derivative and the third zone comprises a stationary polyclonal or monoclonal antibody or antibody fragment capable of binding said caffeine-binding moiety.
A spacer peptide comprising 7-12 amino acids derivatized at its N-terminus to a label or specific binding moiety, which spacer peptide is soluble, temperature-stable, flexible and hydrophilic, lacks internal aromatic, amino, and carboxyl groups;
- and
- comprises a C terminal amino acid that permits binding to a carboxyl-containing or amine-containing compound using diimide chemistry.
Dependencies are inferred from the claim text.