Nucleic acid amplification apparatus and method

A nucleic acid amplification method for amplifying a target nucleic acid derived from living organism, comprising steps of:

• amplifying the target nucleic acid in a first measuring sample prepared from the living organism;
• amplifying the target nucleic acid in a second measuring sample prepared from the living organism and had a difference dilution ratio from the first measurement sample;
• measuring a first product of the amplification of the target nucleic acid of the first measurement sample;
• measuring a second product of the amplification of the target nucleic acid of the second measurement sample;
• obtaining a first measurement value related to an amount of the first product of the amplification;
• obtaining a second measurement value related to an amount of the second product of the amplification;
• and
• judging whether amplification inhibition of the target nucleic acid occur or not based on the first measurement value and the second measurement value.