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US 2007/0178495 A1Published application
Methods and compositions for identifying bacteria associated with bacterial vaginosis
AssigneeFred Hutchinson Cancer Research Center
InventorsDavid Fredricks, Tina Fiedler
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Abstract
The present invention provides methods and compositions for identifying bacteria associated with bacterial vaginosis and diagnosing bacterial vaginosis in a subject.
Claims7
A method of detecting Megasphaera in a sample, comprising:
- a) contacting the sample with a primer pair specific for nucleic acid comprising the nucleotide sequence of SEQ ID NO:64 (Megasphaera) under conditions whereby nucleic acid amplification can occur;
- and
- b) detecting amplification of the nucleic acid of (a), thereby detecting Megasphaera in the sample.
A method of determining the amount of Megasphaera in a sample, comprising:
- a) contacting the sample with a primer pair specific for nucleic acid comprising the nucleotide sequence of SEQ ID NO:64 (Megasphaera) under conditions whereby nucleic acid amplification can occur and the amount of amplified nucleic acid can be determined;
- and
- b) detecting amplification of the nucleic acid of (a) and determining the amount of Megasphaera nucleic acid and the amount of Megasphaera bacteria in the sample.
A method of detecting Megasphaera in a sample, comprising:
- a) contacting the sample with a probe that hybridizes with a nucleic acid comprising the nucleotide sequence of SEQ ID NO:64 (Megasphaera) under conditions whereby nucleic acid hybridization can occur;
- and
- b) detecting nucleic acid hybridization of the probe of (a), thereby detecting Megasphaera in the sample.
Dependencies are inferred from the claim text.