Alterations in the Copy Number of the SULT1A1 Gene
AssigneeMayo Foundation for Medical Education and Research
InventorsRichard Weinshilboum, Araba Adjei, Scott Hebbring, Stephen Thibodeau
Life of this patent
Estimating the term…
Abstract
Methods are described for determining sulfonator status of a patient and determining dosages of drugs based on copy number of the SULT1A1 gene.
Claims27
A method of determining sulfonator status of a patient, said method comprising:
- a) providing a biological sample from said patient;
- b) determining copy number of the SULT1A1 gene in said biological sample;
- and
- c) correlating copy number of the SULT1A1 gene with sulfonator status of said patient.
A method for determining the dosage of a drug to be administered to a patient, wherein said drug is a substrate of SULT1A1, said method comprising:
- a) providing a biological sample from said patient;
- b) determining copy number of the SULT1A1 gene in said biological sample;
- and
- c) determining the dosage of said drug based, at least in part, on the copy number of the SULT1A1 gene.
An article of manufacture comprising a first oligonucleotide primer and a second oligonucleotide primer, wherein the first and second primers, in the presence of mammalian genomic DNA and under polymerase chain reaction conditions, produce a first nucleic acid product corresponding to a region of a SULT1A1 gene and a second nucleic acid product corresponding to a non-polymorphic region of a mammalian genome, and wherein said first and second nucleic acid products are different lengths.
A method of determining copy number of a gene, said method comprising:
- a) providing a biological sample containing mammalian genomic DNA;
- b) producing first and second nucleic acid products from said biological sample using a first oligonucleotide primer and a second oligonucleotide primer under polymerase chain conditions, said first nucleic acid product corresponding to a region of a target gene and said second nucleic acid product corresponding to a non-polymorphic region of a mammalian genome, and wherein said first and second nucleic acid products are different lengths;
- and
- c) determining copy number based on the relative proportion of said first and second nucleic products.
Dependencies are inferred from the claim text.