Methods for detecting modulators of cytokine receptor zalpha11
AssigneeZymoGenetics, Inc.
InventorsJulia Novak, Angela Hammond
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Abstract
Novel polypeptides, polynucleotides encoding the polypeptides, and related compositions and methods are disclosed for zalpha11, a novel cytokine receptor. The polypeptides may be used within methods for detecting ligands that stimulate the proliferation and/or development of hematopoietic, lymphoid and myeloid cells in vitro and in vivo. Ligand-binding receptor polypeptides can also be used to block ligand activity in vitro and in vivo. The polynucleotides encoding zalpha11, are located on chromosome 16, and can be used to identify a region of the genome associated with human disease states. The present invention also includes methods for producing the protein, uses therefor and antibodies thereto.
Claims19
A method of detecting, in a test sample, the presence of a modulator of zalpha11 polypeptide activity, comprising:
- culturing a cell into which has been introduced an expression vector comprising the following operably linked elements:
- a transcription promoter;
- a DNA segment encoding a polypeptide consisting of an amino acid sequence as shown in SEQ ID NO:2 from amino acid number 20 (Cys), to amino acid number 538 (Ser), wherein the polypeptide further comprises a WSXWS motif as shown in SEQ ID NO:3, and wherein a Trp residue in the WSXWS motif (SEQ ID NO:3) is mannosylated;
- and
- a transcription terminator,
- wherein the promoter is operably linked to the DNA segment, and the DNA segment is operably linked to the transcription terminator;
- wherein the cell expresses the polypeptide encoded by the DNA segment in the presence and absence of a test sample;
- and
- comparing levels of activity of the polypeptide in the presence and absence of a test sample, by a biological or biochemical assay;
- and
- determining from the comparison, the presence of the modulator of zalpha11 polypeptide activity in the test sample.
A method of detecting, in a test sample, the presence of a modulator of zalpha11 polypeptide activity, comprising:
- culturing a cell into which has been introduced an expression vector comprising the following operably linked elements:
- a transcription promoter;
- a DNA segment encoding a polypeptide consisting of an amino acid sequence as shown in SEQ ID NO:2 from amino acid number 20 (Cys), to amino acid number 237 (His), wherein the polypeptide further comprises a WSXWS motif as shown in SEQ ID NO:3, and wherein a Trp residue in the WSXWS motif (SEQ ID NO:3) is mannosylated, and wherein the polypeptide further comprises a transmembrane domain and an intracellular domain from a heterologous cytokine receptor;
- and
- a transcription terminator,
- wherein the promoter is operably linked to the DNA segment, and the DNA segment is operably linked to the transcription terminator;
- wherein the cell expresses the polypeptide encoded by the DNA segment in the presence and absence of a test sample;
- and
- comparing levels of activity of the polypeptide in the presence and absence of a test sample, by a biological or biochemical assay;
- and
- determining from the comparison, the presence of the modulator of zalpha11 polypeptide activity in the test sample.
Dependencies are inferred from the claim text.