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US 2007/0178485 A1Published application
Molecular beacons, methods and kits for detecting DNA damage response
AssigneeThe Trustees of the University of Pennsylvania
InventorsWafik El-Deiry, Rishita Shah
Life of this patent
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Abstract
This invention relates to embodiment to a molecular beacon, methods and kits for the detection of expression of p21(Waf1/Cip1), in response to chemotherapy. Specifically, the introduction of a phosphorothioate-modified p21-beacon by transfection in human tumor cells yields increased signal in a dose dependent manner.
Claims28
A molecular beacon comprising:
- an oligonucleotide comprising a stem and a loop structure and having a photoluminescent dye at one of the 5′ or 3′ ends and a quenching agent at an opposite 3′ or 5′ ends, wherein said loop consists of about 13-25 bases and has a melting temperature (Tm) of 52-54° C., and wherein the stem consists of two non-contiguous stretches of base pairs having sequences comprising 5′-CCAAACC (SEQ ID NO.2) and GGTTTG” (SEQ ID NO.3) [respectively].
Dependencies are inferred from the claim text.