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US 2007/0178484 A1Published application
Rapid, sensitive and quantitative methods for tissue and cell-based proteomics via consecutive addition of quantifiable extenders
AssigneeThe Trustees of the University of Pennsylvania
InventorJames Eberwine
Life of this patent
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Abstract
Methods, systems and kits are provided for detecting and quantifying multiple immunogens in a sample via consecutive addition of quantifiable extenders to immunogen bound complexes of immunogen binding agent attached to a DNA containing an RNA polymerase promoter.
Claims2
(canceled)
A kit for detection and quantification of multiples immunogens in a sample via consecutive addition of quantifiable extenders comprising:
- p1 (a) at least two epitope detectors comprising different immunogen binding agents attached to identical double-stranded DNA containing an RNA polymerase and a restriction site overhang;
- (b) oligo 1 comprising a DNA sequence containing the same restriction site overhang on a first strand of oligo 1 as the epitope detectors and a different restriction site overhang on a second strand, said oligo 1 being phosphorylated on the first strand containing the same restriction site as the epitope detector;
- and
- (c) oligo 2 comprising the same DNA sequence as oligo 1, said oligo 2 being phosphorylated on the second strand containing the different restriction site.
1 more element
Dependencies are inferred from the claim text.