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US 2007/0178466 A1Published application
Composition for deaminating dna and method of detecting methylated dna
AssigneeToyo Boseki Kabushiki Kaisha
InventorsHikoya Hayatsu, Kazuo Negishi, Masahiko Shiraishi
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Abstract
(1) A sulfite composition having a sulfite concentration of more than 6.2 M, (2) a method for deaminating DNA using a sulfite composition described in (1), (3) a method for detecting methylated DNA using a sulfite composition described in (1), (4) a kit for deaminating DNA or for detecting methylated DNA comprising a sulfite composition described in (1).
Claims18
A sulfite composition having a sulfite concentration of more than 6.2 M.
A method for deaminating DNA comprising the following steps of:
- (1) treating a sample containing a single-stranded DNA with a sulfite composition having a sulfite concentration of more than 6.2 M;
- and
- (2) treating the sample treated in (1) with an alkali.
A method for detecting methylated DNA comprising the following steps of:
- (a) performing deamination treatment by treating a sample containing a single-stranded DNA with a sulfite composition having a sulfite concentration of more than 6.2 M and treating it with an alkali;
- and
- (b) detecting methylated DNA in the sample obtained in (a).
Dependencies are inferred from the claim text.