Methods for genome amplification
AssigneeHuman Genetic Signatures Pty. Ltd.
InventorDouglas Millar
Life of this patent
Estimating the term…
Abstract
A method for whole genome amplification comprising (a) treating genomic DNA with a modifying agent which modifies cytosine bases but does not modify 5′-methyl-cytosine bases under conditions to form single stranded modified DNA; (b) providing a population of random X-mers of exonuclease-resistant primers capable of binding to at least one strand of the modified DNA, wherein X is an integer 3 or greater; (c) providing polymerase capable of amplifying double stranded DNA, together with nucleotides and optionally any suitable buffers or diluents to the modified DNA; and (d) allowing the polymerase to amplify the modified DNA.
Claims29
A method for whole genome amplification comprising:
- (a) treating genomic DNA with a modifying agent which modifies cytosine bases but does not modify 5′-methyl-cytosine bases under conditions to form single stranded modified DNA;
- (b) providing a population of random X-mers of exonuclease-resistant primers capable of binding to at least one strand of the modified DNA, wherein X is an integer 3 or greater;
- (c) contacting the modified DNA with nucleotides and a polymerase capable of amplifying double stranded DNA;
- and
- (d) allowing the polymerase to amplify the modified DNA.
A population of random X-mers of exonuclease-resistant primers capable of binding to at least one strand of modified DNA in whole genome amplification, where X is an integer of 3 or greater, and wherein the primers are formed of two populations of primers, the first population being random X-mers containing the bases A, G and T, and the second population comprising random X-mers containing the bases A, C and T.
Dependencies are inferred from the claim text.