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AssigneeThe Scripps Research Institute
InventorsMeng-Lin Tsao, Feng Tian, Peter Schultz
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The invention relates to posttranslational modification of phage-displayed polypeptides. These displayed polypeptides comprise at least one unnatural amino acid, e.g., an aryl-azide amino acid such as p-azido-L-phenylalanine, or an alkynyl-amino acid such as para-propargyloxyphenylalanine, which are incorporated into the phage-displayed fusion polypeptide at a selected position by using an in vivo orthogonal translation system comprising a suitable orthogonal aminoacyl-tRNA synthetase and a suitable orthogonal tRNA species. These unnatural amino acids advantageously provide targets for posttranslational modifications such as azide-alkyne [3+2] cycloaddition reactions and Staudinger modifications.
A phage comprising a polypeptide, said polypeptide comprising at least one post-translationally modified unnatural amino acid residue selected from an aryl-azide unnatural amino acid residue and an alkynyl unnatural amino acid residue.
A method for producing a post-translationally modified phage, the method comprising:
A phage comprising a polypeptide, said polypeptide comprising at least one post-translationally modified unnatural amino acid residue.
Dependencies are inferred from the claim text.