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AssigneeBethyl Laboratories, Inc.
InventorsEric McIntush, James Billings, Valarie Florack, Debra Garrett, Henry Carwile
Estimating the term…
Methods and materials are provided for decreasing or eliminating interference from molecules resulting from upstream immunochemical procedures, such as Immunoprecipitation, that employ an Immunoglobulin Binding Molecule (an IBM, e.g., Protein A) in subsequent downstream methods, such as Western Blot. An effective amount of a ligand of the IBM is used to block the IBM or fragments of the IBM on a support membrane used in the downstream method. In another embodiment, a secondary antibody that is specific either for heavy chain or light chain antibody fragments or that recognizes intact antibody molecules but does not recognize individual antibody fragments may be employed with the ligand of the IBM.
In an immunochemical analytical method having an upstream procedure that employs an immunoglobulin binding molecule (IBM) to produce a product of the upstream procedure and a downstream procedure to treat the product of the upstream procedure, the step of adding a ligand of the IBM to block a binding site of the IBM as a part of the downstream procedure.
A method of analysis for a protein solution having a protein of interest, comprising:
A method for identifying ligands to be used to block immunoglobulin binding molecules and fragments thereof comprising using the IBM that is used in an upstream immunochemical method in a method that identifies molecules that compete for the immunoglobulin binding site on an IBM.
An article of manufacture comprising:
Dependencies are inferred from the claim text.