Measuring contamination
AssigneeMycometer ApS
InventorsMorton Reeslev, Morten Miller
Life of this patent
Estimating the term…
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AssigneeMycometer ApS
InventorsMorton Reeslev, Morten Miller
Estimating the term…
Disclosed is a convenent sample preparation method for a medium suspected of containing contaminants, the method comprising a) passing a known volume of said medium through a filter from an influent side to an effluent side thereby concentrating the contaminants on the influent side of the filter, b) contacting the influent side of the filter with a liquid vehicle containing at least one substrate that through interaction with the contaminants each produces a detectable moiety, c) and allowing the substrate to interact with the contaminants on the influent side of the filter for a period of time, which is sufficient to allow the detectable moiety to be detected in the liquid vehicle. The method may further comprise a detection step, where the amount of detectable is determined in the liquid vehicle, preferably after the liquid vehicle has been separated from the contaminant, e.g. by passing the liquid vehicle through the filter and performing a measurement on the contaminant free liquid vehicle. Also disclosed is a kit for exercising the inventive method.
A sample preparation method for a medium suspected of containing contaminants, the method comprising a) passing a known volume of said medium through a filter from an influent side to an effluent side in a filter device thereby concentrating the contaminants on the influent side of the filter in the filter device, b) contacting the influent side of the filter in the filter device with a liquid vehicle containing at least one substrate that through interaction with the contaminants each produces a detectable moiety, c) and allowing the substrate to interact with the contaminants on the influent side of the filter in the filter device for a period of time, which is sufficient to allow the detectable moiety to be detected in the liquid vehicle.
The method according to 36, wherein the correlation in step d comprises the use of a pre-determined standard curve that expresses the relationship between the amount of contaminants and the amount of the detectable moiety under standard conditions.
A kit for determination of contaminants in a medium, the kit comprising
Use of a closed, sterile filter device as a reaction vessel for a reaction between contaminants retained in the device and a substrate that releases a detectable moiety when contacted with the contaminants.
Dependencies are inferred from the claim text.