Screening method for a ribonuclease h in hibitor of a reverse transcriptase

A screening method for an RNase H inhibitor of a reverse transcriptase, comprising;

• (a) a process to incubate a substrate which a primer hybridized to a template, a metal ion and a reverse transcriptase to form a complex,
• (b) a process to add a test substance after the process (a) and incubate, and
• (c) a process to add dNTPs after process (b) to initiate DNA synthesis, provided that,
• the template is 5′-NRWXZ-3′ and the primer is 3′-Y-5′ (Y hybridizes to X of a template),
• the template is 5′-NRWX-3′ and the primer is 3′-YZ-5′ (Y hybridizes to X of a template),
• or the substrate is 5′-NRWXZY-3′ (Y hybridizes to X),
• wherein,
• N is 13-19 mer DNA, RNA or a chimeric nucleic acid,
• R is RNA,
• W is DNA or a chimeric nucleic acid,
• X is 15 mer or more DNA, RNA or a chimeric nucleic acid,
• Y is a same length DNA, RNA or chimeric nucleic acid with X to which Y hybridizes.
• In case that X to which Y hybridizes is DNA, Y is DNA. In case that X to which Y hybridizes is RNA, Y is RNA. In case that X to which Y hybridizes is a chimeric nucleic acid, Y is a chimeric nucleic acid (In the chimeric nucleic acid, in case that X to which Y hybridizes is DNA, Y is DNA. In case that X to which Y hybridizes is RNA, Y is RNA).
• Z is DNA, RNA or a chimeric nucleic acid
• (provided that, W and Z can be absent).