Composition for maintaining organ and cell viability

A method of preserving a mammalian heart, ex vivo, comprising contacting or perfusing a mammalian heart with an effective amount of an aqueous composition that comprises:

• a first phase comprising a base nutritive medium;
• and
• a second phase comprising nanoparticles that comprise an outer lipophilic coating and an inner hydrophilic core, wherein
• a) the first phase comprises physiologically compatible concentrations of water soluble or dispersible nutrients, and physiological salts;
• b) the nanoparticles of the second phase comprise lipids, fatty acids, sterols and free fatty acids;
• and
• c) the two-phase composition has an osmolality of at least about 300 mOsM/kg, and
• wherein the nanoparticles have a mean diameter ranging from about 100 nm to about 300 nm, and wherein the hydrophilic inner core of the nanoparticle contains a solution or suspension comprising a moiety capable of binding and releasing oxygen, and the first phase comprises a composition of Table 2A, wherein Table 2A is:
• g/Liter
• RANGE
• Adenosine
• 0.950-1.050
• Adenosine 5′ Monophosphate
• 0.0019-0.0021
• Adenosine Triphosphate
• 0.0019-0.0021
• Allopurinol
• 0.133-0.147
• B′ Nicotinamide Adenine Dinucleotide
• 0.038-0.042
• Phosphate
• B′Nicotinamide Adenine Dinucleotide
• 0.0019-0.0021
• Calcium Chloride
• 0.152-0.168
• Choline Chloride
• 0.0085-0.0094
• Chondrotin Sulfate
• 0.0038-0.0042
• Cocarboxylase
• 0.038-0.042
• Coenzyme A
• 0.0095-0.00105
• Cyclodextrin
• 0.475-0.525
• Deoxyadenosine
• 0.038-0.042
• Deoxycytidine
• 0.038-0.042
• Deoxyguanosine
• 0.038-0.042
• Dextran 70
• 33.25-36.75
• Flavin Adenine Dinucleotide
• 0.038-0.042
• Folic Acid
• 0.0026-0.0028
• Glucose
• 3.800-4.200
• Glutathione
• 0.950-1.050
• Glycine
• 0.0179-0.0197