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US 2007/0178074 A1Published application
Chondrocyte Culture Formulations
OwnerDan-Ning Hu, Steven McCormickinventors
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OwnerDan-Ning Hu, Steven McCormickinventors
Estimating the term…
The present invention provides methods and compositions for providing graft recipients with chondrocytes. Specifically, the methods and compositions of the invention provide for populations of chondrocytes that may be isolated from a patient and cultured in vitro to generate a proliferating population of chondrocytes that exhibit great proliferation capacity. The invention is based, in part, on the discovery of a culture medium having serum, insulin and various growth factors for culturing chondrocytes that exhibit increased proliferative capacity. The invention provides novel in vitro methods for culturing chondrocytes, including those isolated from the cartilage of a healthy subject, to generate a proliferating population of chondrocytes. The methods and compositions of the invention may be used for transplantation to treat patients having damaged cartilage.
A composition for culturing a population of chondrocytes comprising basal medium, serum, antibiotic, hormone, and growth factor, wherein the growth factor comprises PDGF and HGF.
The method of claim 12, wherein the cultured chondrocytes are applied to surface of, or in the local environment of the damaged articular cartilage.
A composition comprising a population of chondrocytes cultured in a medium comprising basal medium, serum, antibiotic, hormone, and growth factor, wherein the growth factor comprises PDGF and HGF, wherein the cultured chondrocytes exhibit a number of divisions before senescence, wherein the number of divisions is at least 10.
A composition for culturing a population of chondrocytes comprising Ham's F12, fetal bovine serum, glutamine, gentamicin, insulin, and growth factor, wherein the growth factor comprises bFGF, PDGF and HGF.
Dependencies are inferred from the claim text.