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US 2007/0177143 A1Published application
Process and device for determination of cell viability
OwnerJoseph Chaiken, Robert Dracker +2inventors
InventorsJoseph Chaiken, Robert Dracker, Pamela Hagrman, Douglas Hagrman
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Abstract
Described is a method to determine the viability of cells by measuring the absolute and relative rate of metabolic activity and/or integrity of the cell membrane through the use of vibrational spectroscopy. The use of deuterated agents facilitates detection of changes associated with a change in viability.
Claims16
A method of determining cell viability, comprising:
- (a) obtaining a container loaded with deuterated materials;
- (b) introducing cells into the container whereby the cells are in contact with the deuterated materials;
- (c) obtaining vibrational spectra emitted by the cells;
- wherein the vibrational spectra emitted by the cells are indicative of metabolism, thereby providing an indication of viability of the cells, such that greater metabolic activity is indicative of greater viability.
1 more element
A method of determining cell viability, comprising:
- (a) obtaining a container loaded with deuterated materials;
- (b) introducing cells into the container whereby the cells are in contact with the deuterated materials;
- (c) obtaining vibrational spectra emitted by the cells;
- (d) placing an aliquot of the cells into a medium free of deuterated materials;
- (e) obtaining vibrational spectra emitted by the cells in the non-deuterated medium;
- (f) determining the rate of decrease of emitted vibrational spectra;
- wherein the vibrational spectra are indicative of metabolism, thereby providing an indication of viability of the cells, such that a faster rate of decrease of emitted vibrational spectra is indicative of greater viability.
4 more elements
Dependencies are inferred from the claim text.