Optimized sample injection structures in microfluidic separations
AssigneeIntegenX Inc.
InventorsMichael Nguyen, Luc Bousse
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Abstract
The invention herein provides improved sample injection systems and related methods to create microfluidic devices with symmetrical channel configurations that can produce relatively large sample volumes. An embodiment of the invention provides microfluidic structures with different geometries that are symmetrical from the perspective of a sample load channel and a sample waste channel, which essentially eliminates issues of time offset and other problems commonly associated with twin-T sample formation techniques. A split-injection approach and related methods of sample plug formation are therefore provided.
Claims19
A microfluidic device for sample injection which comprises
- a sample channel, which contains a sample having an original sample composition, a separation channel, and two buffer channels, which contain an electrolyte buffer,
- wherein the buffer and separation channels each intersect the sample channel, and the separation channel is positioned between the buffer channels, such that a sample volume is substantially defined by a section of the sample channel between the outermost boundaries of the two buffer channels where they intersect the sample channel.
A method of introducing a sample into a microfluidic device, which microfluidic device comprising
- a sample channel connected to a sample well and a waste well at the two separate ends, where the sample well contains a sample having an original sample composition, two buffer channels, which contain an electrolyte buffer, and a separation channel,
- wherein the buffer and separation channels are each inclined with respect to the sample channel, and the separation channel is between the buffer channels,
- and wherein buffer and separation channels intersect the sample channel, such that a geometrically defined sample chamber is a sample volume defined by a section of the sample channel located between the outermost boundaries of the two buffer channels where they intersect the sample channel,
- which method comprises the step of electrokinetically loading a sample into the sample channel by applying an electric field across the sample well and the waste well, wherein the electric field is applied for a time period which is at least long enough that the component of the sample having the lowest electrophoretic mobility migrates into the geometrically defined sample volume, such that the loaded sample plug reflects the original sample composition.
Dependencies are inferred from the claim text.