Altering levels of anti-nutrient factors in plants

The method of claim 21, wherein the two or more than two enzymes are selected from the group consisting of IP3K-IP6K and PIKa-PIKb.

A construct comprising a silencing nucleotide sequence, the silencing nucleotide sequence encoding two or more than two sequences that reduce or inhibit the expression of two or more that two enzymes involved in the synthesis of the one or more than one protein leading to sinapine biosynthesis, lignin biosynthesis, phytate biosynthesis, or a combination thereof.

A plant comprising a nucleic acid sequence comprising a regulatory region operatively associated with a silencing nucleotide sequence, the silencing nucleotide sequence reducing or eliminating the expression of two or more than two enzymes involved in the synthesis of the one or more than one protein leading to sinapine biosynthesis, lignin biosynthesis, phytate biosynthesis, or a combination thereof.

A method for reducing the level of one or more than one protein within a plant or a tissue within the plant comprising, expressing a nucleotide sequence within the plant or a tissue within the plant, the nucleic acid sequence comprising a regulatory region operatively associated with a silencing nucleotide sequence, where expression of the silencing nucleotide sequence reduces or eliminates the expression of two or more than two enzymes involved in the synthesis of the one or more than one protein leading to sinapine biosynthesis, lignin biosynthesis, phytate biosynthesis, or a combination thereof, the reduced level of the one or more than one protein determined by comparing the level of the protein in the plant, or a tissue of the plant, with a level of the protein in a second plant, or the tissue from the second plant, that does not express the nucleic acid sequence.

A seed comprising a silencing nucleic acid sequence and having a reduced level of one or more than one anti-nutrient compound, when compared to the level of the one or more anti-nutrient compound in a wild type seed.

A method for reducing the level of one or more than one protein in a plant or a tissue within the plant comprising,

• i) providing a plant comprising a nucleic acid sequence, the nucleic acid sequence comprising a regulatory region operatively associated with a silencing nucleotide sequence, wherein expression of the silencing nucleotide sequence reduces or eliminates the expression of two or more than two enzymes involved in the synthesis of the one or more than one protein, and
• ii) expressing the silencing nucleotide sequence within the plant or a tissue within the plant, to reduce the level of the one or more than one protein in the plant or within a tissue of the plant, the reduced level of the one or more than one protein is determine by comparing the level of the one or more than one protein in the plant, or a tissue of the plant, with a level of the one ore more than one protein in a second plant, or the tissue from the second plant, that does not express the silencing nucleic acid sequence.