Methods and Compositions for Modifying Flower Development
AssigneePioneer Hi-Bred International, Inc.
InventorsWesley Bruce, Suling Zhao
Life of this patent
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Abstract
The present invention provided methods and compositions for modifying flower development and reproductive development in plants. The methods involve transforming a plant with a polynucleotide construct comprising a promoter operably lined to polynucleotide molecule encoding a short vegetative phase-like transcription factor. The compositions include transformed plants, plant cells, seeds, and expression cassettes comprising a polynucleotide molecule encoding a short vegetative phase-like transcription factor or fragment or variant thereof.
Claims72
A method for modifying flower development in a plant, said method comprising transforming a plant with a polynucleotide construct comprising a polynucleotide molecule operably linked to a promoter that is expressed in a plant cell, wherein the polynucleotide molecule comprises a nucleotide sequence selected from the group consisting of:
- (a) the nucleotide sequence set forth in SEQ ID NO:
- 1, 3 or 4;
- (b) the nucleotide sequence set forth in GenBank Accession Number AJ430635;
- (c) a nucleotide sequence encoding an amino acid sequence comprising SEQ ID NO:
- 2;
- (d) a nucleotide sequence comprising at least 90% sequence identity to SEQ ID NO:
- 1 or 3, wherein said polynucleotide molecule encodes a polypeptide having ZmSVP activity;
- (e) a nucleotide sequence that hybridizes under stringent conditions to the complement of the nucleotide sequence of (a) or (b), wherein said stringent conditions comprise hybridization in 50% formamide, 1 M NaCl, 1% SDS at 37° C., and a wash in 0.1×SSC at 60° C. to 65° C., wherein said polynucleotide molecule encodes a polypeptide having ZmSVP activity;
- (f) a nucleotide sequence comprising at least 250 consecutive nucleotides of SEQ ID NO:
- 1 or 3 or a complement thereof;
- (g) a nucleotide sequence encoding an amino acid sequence having at least 90% sequence identity to SEQ ID NO:2, wherein said polynucleotide molecule encodes a polypeptide having ZmSVP activity;
- and
- (h) a nucleotide sequence that is the complement of the nucleotide sequence of (a), (b), (c), (d), (e), (f) or (g);
- wherein flower development is modified in the transformed plant when compared to an untransformed plant.
A method for modifying reproductive development in a plant, said method comprising transforming a plant with a polynucleotide construct comprising a polynucleotide molecule operably linked to a promoter that is expressed in a plant cell, wherein the polynucleotide molecule comprises a nucleotide sequence selected from the group consisting of:
- (a) the nucleotide sequence set forth in SEQ ID NO:
- 1, 3 or 4;
- (b) the nucleotide sequence set forth in GenBank Accession Number AJ430635;
- (c) a nucleotide sequence encoding an amino acid sequence comprising SEQ ID NO:
- 2;
- (d) a nucleotide sequence comprising at least 90% sequence identity to SEQ ID NO:
- 1 or 3, wherein said polynucleotide molecule encodes a polypeptide having ZmSVP activity;
- (e) a nucleotide sequence that hybridizes under stringent conditions to the complement of the nucleotide sequence of (a) or (b), wherein said stringent conditions comprise hybridization in 50% formamide, 1 M NaCl, 1% SDS at 37° C., and a wash in 0.1×SSC at 60° C. to 65° C., wherein said polynucleotide molecule encodes a polypeptide having ZmSVP activity;
- (f) a nucleotide sequence comprising at least 250 consecutive nucleotides of SEQ ID NO:
- 1 or 3 or a complement thereof;
- (g) a nucleotide sequence encoding an amino acid sequence having at least 90% sequence identity to SEQ ID NO:
- 2, wherein said polynucleotide molecule encodes a polypeptide having ZmSVP activity;
- and
- (h) a nucleotide sequence that is the complement of the nucleotide sequence of (a), (b), (c), (d), (e), (f) or (g);
- wherein reproductive development is modified in the transformed plant when compared to an untransformed plant.
A method for increasing the grain yield of a grain plant comprising, said method comprising transforming a plant with a polynucleotide construct comprising a polynucleotide molecule operably linked to a promoter that is expressed in a plant cell, wherein the polynucleotide molecule comprises a nucleotide sequence selected from the group consisting of:
- (a) the nucleotide sequence set forth in SEQ ID NO:
- 1, 3 or 4;
- (b) the nucleotide sequence set forth in GenBank Accession Number AJ430635;
- (c) a nucleotide sequence encoding an amino acid sequence comprising SEQ ID NO:
- 2;
- (d) a nucleotide sequence comprising at least 90% sequence identity to SEQ ID NO:
- 1 or 3, wherein said polynucleotide molecule encodes a polypeptide having ZmSVP activity;
- (e) a nucleotide sequence that hybridizes under stringent conditions to the complement of the nucleotide sequence of (a) or (b), wherein said stringent conditions comprise hybridization in 50% formamide, 1 M NaCl, 1% SDS at 37° C., and a wash in 0.1×SSC at 60° C. to 65° C., wherein said polynucleotide molecule encodes a polypeptide having ZmSVP activity;
- (f) a nucleotide sequence comprising at least 250 consecutive nucleotides of SEQ ID NO:
- 1 or 3 or a complement thereof;
- (g) a nucleotide sequence encoding an amino acid sequence having at least 90% sequence identity to SEQ ID NO:
- 2, wherein said polynucleotide molecule encodes a polypeptide having ZmSVP activity;
- and
- (h) a nucleotide sequence that is the complement of the nucleotide sequence of (a), (b), (c), (d), (e), (f) or (g);
- wherein the grain yield of the transformed grain plant is increased when compared to the grain yield of an untransformed grain plant grown.
A transformed plant comprising a polynucleotide construct comprising a polynucleotide molecule operably linked to a promoter that drives expression in the plant, wherein said polynucleotide molecule comprises a nucleotide sequence selected from the group consisting of:
- (a) the nucleotide sequence set forth in SEQ ID NO:
- 1, 3 or 4;
- (b) the nucleotide sequence set forth in GenBank Accession Number AJ430635;
- (c) a nucleotide sequence encoding an amino acid sequence comprising SEQ ID NO:
- 2;
- (d) a nucleotide sequence comprising at least 90% sequence identity to SEQ ID NO:
- 1 or 3, wherein said polynucleotide molecule encodes a polypeptide having ZmSVP activity;
- (e) a nucleotide sequence that hybridizes under stringent conditions to the complement of the nucleotide sequence of (a) or (b), wherein said stringent conditions comprise hybridization in 50% formamide, 1 M NaCl, 1% SDS at 37° C., and a wash in 0.1×SSC at 60° C. to 65° C., wherein said polynucleotide molecule encodes a polypeptide having ZmSVP activity;
- (f) a nucleotide sequence comprising at least 250 consecutive nucleotides of SEQ ID NO:
- 1 or 3 or a complement thereof;
- (g) a nucleotide sequence encoding an amino acid sequence having at least 90% sequence identity to SEQ ID NO:
- 2, wherein said polynucleotide molecule encodes a polypeptide having ZmSVP activity;
- and
- (h) a nucleotide sequence that is the complement of the nucleotide sequence of (a), (b), (c), (d), (e), (f) or (g).
A transformed plant cell comprising a polynucleotide construct comprising a polynucleotide molecule operably linked to a promoter that drives expression in the plant, wherein said polynucleotide molecule comprises a nucleotide sequence selected from the group consisting of:
- (a) the nucleotide sequence set forth in SEQ ID NO:
- 1, 3 or 4;
- (b) the nucleotide sequence set forth in GenBank Accession Number AJ430635;
- (c) a nucleotide sequence encoding an amino acid sequence comprising SEQ ID NO:
- 2;
- (d) a nucleotide sequence comprising at least 90% sequence identity to SEQ ID NO:
- 1 or 3, wherein said polynucleotide molecule encodes a polypeptide having ZmSVP activity;
- (e) a nucleotide sequence that hybridizes under stringent conditions to the complement of the nucleotide sequence of (a) or (b), wherein said stringent conditions comprise hybridization in 50% formamide, 1 M NaCl, 1% SDS at 37° C., and a wash in 0.1×SSC at 60° C. to 65° C., wherein said polynucleotide molecule encodes a polypeptide having ZmSVP activity;
- (f) a nucleotide sequence comprising at least 250 consecutive nucleotides of SEQ ID NO:
- 1 or 3 or a complement thereof;
- (g) a nucleotide sequence encoding an amino acid sequence having at least 90% sequence identity to SEQ ID NO:
- 2, wherein said polynucleotide molecule encodes a polypeptide having ZmSVP activity;
- and
- (h) a nucleotide sequence that is the complement of the nucleotide sequence of (a), (b), (c), (d), (e), (f) or (g).
An isolated polynucleotide molecule comprising a nucleotide sequence selected from the group consisting of:
- (a) the nucleotide sequence set forth in SEQ ID NO:
- 1, 3 or 4;
- (b) a nucleotide sequence encoding an amino acid sequence comprising SEQ ID NO:
- 2;
- and
- (c) a nucleotide sequence that is the complement of the nucleotide sequence of (a) or (b).
An isolated polypeptide comprising an amino acid sequence selected from the group consisting of:
- (a) the amino acid sequence set forth in SEQ ID NO:
- 2;
- and
- (b) the amino acid sequence encoded by the nucleotide sequence set forth in SEQ ID NO:
- 1, 3 or 4.
A method for producing a maize plant comprising altered flower development, said method comprising the steps of:
- (a) transforming a maize plant cell with at least one expression cassette capable of expressing a polynucleotide that reduces or eliminates the activity of the expression of the ZmSVP protein in a maize plant or part thereof;
- and
- (b) regenerating a transformed maize plant from the transformed maize plant cell of step (a).
A maize plant that is genetically modified to alter flower development, wherein the maize plant is genetically modified to reduce or eliminate the activity of the ZmSVP protein.
Dependencies are inferred from the claim text.