Novel vector and method of constructing transformant plant using the vector
AssigneeNippon Paper Industries Co., Ltd.
InventorsAkiyoshi Kawaoka, Kazuya Nanto, Hiroyasu Ebinuma
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AssigneeNippon Paper Industries Co., Ltd.
InventorsAkiyoshi Kawaoka, Kazuya Nanto, Hiroyasu Ebinuma
Estimating the term…
Gene introduction to a plant is carried out by using a vector which comprises the following constitutional units A and B: A: a DNA sequence comprising P1, an expression inhibitory sequence and a desired gene which is controlled by P1 and the expression inhibitory sequence; B: a DNA sequence comprising P2, an expression inhibitory gene which is controlled by P2, P3, the expression inhibitory sequence, and a gene of a removal reaction-catalyzing enzyme which is controlled by P3 and the expression inhibitory sequence, wherein the DNA sequence is removed by expression of the gene of a removal reaction-catalyzing enzyme, wherein P1 is a promoter which shows its activity in at least a callus and a plant tissue where a desired gene should be expressed, P2 is a promoter which shows its activity in at least a callus, and P3 is a promoter which shows its activity in at least a plant tissue where P1 and P2 do not show their activities.
A vector which comprises the following constitutional units A and B positioned on the same DNA molecule or on different DNA molecules, respectively, said vector comprising:
Dependencies are inferred from the claim text.