Nucleotide sequences and corresponding polypeptides conferring improved nitrogen use efficiency characteristics in plants
OwnerGreg Nadzan, Richard Schneeberger +1inventors
InventorsGreg Nadzan, Richard Schneeberger, Kenneth Feldmann
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Abstract
The present invention relates to isolated nucleic acid molecules and their corresponding encoded polypeptides able to confer the traits of improved nitrogen use efficiency in plants. The present invention further relates to the use of these nucleic acid molecules and polypeptides in making transgenic plants, plant cells, plant materials or seeds of a plant having improved nitrogen use efficiency that leads to improvement in plant size, vegetative growth, growth rate, seedling vigor and/or biomass that are altered with respect to wild type plants grown under normal and/or abnormal nitrogen conditions.
Claims24
A method of improving nitrogen use efficiency, modulating vegetative growth, seedling vigor and/or plant biomass, said method comprising introducing into a plant cell an isolated nucleic acid comprising a nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence that encodes an amino acid sequence that is at least 85% identical to any one of Leads 82, 85, 92, 93, 98, 112, ME07344, ME05213, ME02730 and ME24939, SEQ ID NOs:
- 81, 105, 107, 114, 116, 201, 140, 84, 112 and 200, respectively;
- (b) a nucleotide sequence that is complementary to any one of the nucleotide sequences according to paragraph (a);
- (c) a nucleotide sequence according to any one of SEQ ID NOs:
- 80, 104, 106, 113, 115, 127, 139, 202, 203 and 204;
- (d) a nucleotide sequence that is an interfering RNA to the nucleotide sequence according to paragraph (a);
- (e) a nucleotide sequence able to form a hybridized nucleic acid duplex with the nucleic acid according to any one of paragraphs (a)-(d) at a temperature from about 40° C. to about 48° C. below a melting temperature of the hybridized nucleic acid duplex;
- (f) a nucleotide sequence encoding any one of the amino acid sequences identified as Leads 82, 85, 92, 93, 98, 112, ME07344, ME05213, ME02730 and ME24935, corresponding to SEQ ID NOs:
- 81, 105, 107, 114, 116, 201, 140, 84, 112 and 200, respectively;
- or
- (g) a nucleotide sequence encoding any one of the lead, functional homolog or consensus sequences in FIGS. 1-5, wherein said plant produced from said plant cell has improved nitrogen use efficiency, modulated plant size, modulated vegetative growth, modulated seeding vigor and/or modulated biomass as compared to the corresponding level in tissue of a control plant that does not comprise said nucleic acid.
A plant cell comprising an isolated nucleic acid comprising a nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence encoding an amino acid sequence that is at least 85% identical to any one of Leads 82, 85, 92, 93, 98, 112, ME07344, ME05213, ME02730 and ME24939, corresponding to SEQ ID NOs:
- 81, 105, 107, 114, 116, 201, 140, 84, 112 and 120, respectively;
- (b) a nucleotide sequence that is complementary to any one of the nucleotide sequences according to paragraph (a);
- (c) a nucleotide sequence according to any one of SEQ ID NOs:
- 80, 104, 106, 113, 115, 127, 139, 202, 203 and 204;
- (d) a nucleotide sequence that is an interfering RNA to the nucleotide sequence according to paragraph (a);
- (e) a nucleotide sequence able to form a hybridized nucleic acid duplex with the nucleic acid according to any one of paragraphs (a)-(c) at a temperature from about 40° C. to about 48° C. below a melting temperature of the hybridized nucleic acid duplex;
- (f) a nucleotide sequence encoding any one of the amino acid sequences identified as Leads 82, 85, 92, 93, 98, 112, ME07344, ME05213, ME02730 and ME24935, corresponding to SEQ ID NOs:
- 81, 105, 107, 114, 116, 201, 140, 84, 112 and 200, respectively;
- or
- (g) a nucleotide sequence encoding any one of the lead, functional homolog or consensus sequences in FIGS. 1-5.
A method for improving nitrogen use efficiency and/or the biomass of a plant, said method comprising altering the level of expression in said plant of a nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence that encodes an amino acid sequence that is at least 85% identical to any one of Leads 82, 85, 92, 93, 98, 112, ME07344, ME05213, ME02730 and ME24939, SEQ ID NOs:
- 81, 105, 107, 114, 116, 201, 140, 84, 112 and 200, respectively;
- (b) a nucleotide sequence that is complementary to any one of the nucleotide sequences according to paragraph (a);
- (c) a nucleotide sequence according to any one of SEQ ID NOs:
- 80, 104, 106, 113, 115, 127, 139,202,203and204;
- (d) a nucleotide sequence that is an interfering RNA to the nucleotide sequence according to paragraph (a);
- (e) a nucleotide sequence able to form a hybridized nucleic acid duplex with the nucleic acid according to any one of paragraphs (a)-(d) at a temperature from about 40° C. to about 48° C. below a melting temperature of the hybridized nucleic acid duplex;
- (f) a nucleotide sequence encoding any one of the amino acid sequences identified as Leads 82, 85, 92, 93, 98, 112, ME07344, ME05213, ME02730 and ME24935, corresponding to SEQ ID NOs:
- 81, 105, 107, 114, 116, 201, 140, 84, 112 and 200, respectively;
- or
- (g) a nucleotide sequence encoding any one of the lead, functional homolog or consensus sequences in FIGS. 1-5, wherein said plant produced from said plant cell has improved nitrogen use efficiency, modulated plant size, modulated vegetative growth, modulated seeding vigor and/or modulated biomass as compared to the corresponding level in tissue of a control plant that does not comprise said nucleic acid.
A method for promoting improved nitrogen use efficiency and/or increased biomass in a plant, comprising:
- (a) transforming a plant with a nucleic acid molecule comprising a nucleotide sequence encoding any one of the lead, functional homolog or consensus sequences in FIGS. 1-5;
- and
- (b) expressing said nucleotide sequence in said transformed plant, whereby said transformed plant has an increased nitrogen use efficiency and/or biomass or enhanced seedling vigor as compared to a plant that has not been transformed with said nucleotide sequence.
An isolated nucleic acid molecule comprising:
- (a) a nucleotide sequence that encodes an amino acid sequence that is at least 85% identical to any one of Leads 82, 85, 92, 93, 98, 112, ME07344, ME05213, ME02730 and ME24939, SEQ ID NOs:
- 81, 105, 107, 114, 116, 201, 140, 84, 112 and 200, respectively;
- (b) a nucleotide sequence that is complementary to any one of the nucleotide sequences according to paragraph (a);
- (c) a nucleotide sequence according to any one of SEQ ID NOs:
- 80, 104, 106, 113, 115, 127, 139,202,203and204;
- (d) a nucleotide sequence that is an interfering RNA to the nucleotide sequence according to paragraph (a);
- (e) a nucleotide sequence able to form a hybridized nucleic acid duplex with the nucleic acid according to any one of paragraphs (a)-(c) at a temperature from about 40° C. to about 48° C. below a melting temperature of the hybridized nucleic acid duplex;
- (f) a nucleotide sequence encoding any one of the amino acid sequences identified as Leads 82, 85, 92, 93, 98, 112, ME07344, ME05213, ME02730 and ME24935, corresponding to SEQ ID NOs:
- 81, 105, 107, 114, 116, 201, 140, 84, 112 and 200, respectively;
- or
- (g) a nucleotide sequence encoding any one of the lead, functional homolog or consensus sequences in FIGS. 1-5.
Dependencies are inferred from the claim text.