EPSP synthase domains conferring glyphosate resistance
AssigneeAthenix Corporation
InventorsBrian Carr, Philip Hammer, Todd Hinson, Brian Vande Berg
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Abstract
Compositions and methods for conferring tolerance to glyphosate in bacteria, plants, plant cells, tissues and seeds are provided. Compositions include novel EPSP synthase enzymes and nucleic acid molecules encoding such enzymes, vectors comprising those nucleic acid molecules, and host cells comprising the vectors. The novel proteins comprise at least one sequence domain selected from the domains provided herein. These sequence domains can be used to identify EPSP synthases with glyphosate resistance activity.
Claims20
An isolated polynucleotide other than the polynucleotide of SEQ ID NO:1, 3, 5, 11, 13, 38 or 40 encoding an EPSP synthase polypeptide having a Q-loop, said Q-loop comprising an amino acid sequence with an increased polarity, wherein said polypeptide is resistant to glyphosate.
A method of producing genetically transformed plants which are tolerant toward glyphosate herbicide, comprising the steps of:
- a) inserting into the genome of a plant cell a polynucleotide other than the polynucleotide of SEQ ID NO:1, 13 or 38 encoding a polypeptide having a Q-loop, said Q-loop comprising an amino acid sequence with an increased polarity;
- b) obtaining a transformed plant cell;
- and,
- c) regenerating from the transformed plant cell a genetically transformed plant which has increased tolerance to glyphosate herbicide.
A glyphosate tolerant plant cell comprising a heterologous polynucleotide other than the polynucleotide of SEQ ID NO:1, 13 or 38 encoding an EPSP synthase polypeptide having a Q-loop, said Q-loop comprising an amino acid sequence with an increased polarity, wherein said polypeptide is resistant to glyphosate.
A method for selectively controlling weeds in a field containing a plant having planted seeds or plants comprising the steps of:
- a) planting the seeds or plants which are glyphosate tolerant as a result of a polynucleotide other than the polynucleotide of SEQ ID NO:1, 13, or 38 being inserted into the seed or plant, said polynucleotide having a Q-loop, said Q-loop comprising an amino acid sequence with an increased polarity;
- and,
- b) applying to the plants and weeds in a field an effective concentration of glyphosate herbicide to control weeds without significantly affecting the plants.
Dependencies are inferred from the claim text.