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US 2007/0169217 A1Published application
Geranylgeranyl pyrophosphate synthases
OwnerRebecca Cahoon, Jennie Shen +1inventors
InventorsRebecca Cahoon, Jennie Shen, Mark Williams
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Abstract
This invention relates to an isolated nucleic acid fragment encoding a geranylgeranyl pyrophosphate synthase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the geranylgeranyl pyrophosphate synthase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the geranylgeranyl pyrophosphate synthase in a transformed host cell.
Claims16
An isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of:
- (a) a first nucleotide sequence encoding a polypeptide of at least 35 amino acids that has at least 80% identity based on the Clustal method of alignment when compared to a polypeptide of SEQ ID NO:
- 10;
- (b) a second nucleotide sequence encoding a polypeptide of at least 50 amino acids that has at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NO:6 and SEQ ID NO:42;
- (c) a third nucleotide sequence encoding a polypeptide of at least 50 amino acids that has at least 90% identity based on the Clustal method of alignment when compared to SEQ ID NO:
- 2;
- (d) a fourth nucleotide sequence encoding a polypeptide of at least 100 amino acids that has at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NO:8, SEQ ID NO:12, SEQ ID NO:28, SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:34, SEQ ID NO:36, SEQ ID NO:40, and SEQ ID NO:44;
- (e) a fifth nucleotide sequence encoding a polypeptide of at least 100 amino acids that has at least 85% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NO:4, SEQ ID NO:14, and SEQ ID NO:20;
- (f) a sixth nucleotide sequence encoding a polypeptide of at least 100 amino acids that has at least 90% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NO:16 and SEQ ID NO:26;
- (g) a seventh nucleotide sequence encoding a polypeptide of at least 150 amino acids that has at least 80% identity based on the Clustal method of alignment when compared to SEQ ID NO:24;
- (h) an eighth nucleotide sequence encoding a polypeptide of at least 150 amino acids that has at least 90% identity based on the Clustal method of alignment when compared to SEQ ID NO:22;
- (i) a ninth nucleotide sequence encoding a polypeptide of at least 200 amino acids that has at least 80% identity based on the Clustal method of alignment when compared to SEQ ID NO:38;
- (j) a tenth nucleotide sequence encoding a first polypeptide of at least 200 amino acids that has at least 90% identity based on the Clustal method of alignment when compared to SEQ ID NO:18;
- and
- (k) an eleventh nucleotide sequence comprising the complement of (a), (b), (c), (d), (e), (f), (g), (h), (i), or (j).
11 more elements
2-16. (canceled)
An isolated polynucleotide comprising:
- (a) a nucleotide sequence encoding a polypeptide having geranylgeranyl pyrophosphate synthase activity, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:26 have at least 85% sequence identity, or
- (b) the complement of the nucleotide sequence, wherein the complement and the nucleotide sequence contain the same number of nucleotides and are 100% complementary.
Dependencies are inferred from the claim text.