Thiol methyltransferase-based selection
AssigneeValorisation-Recherche, Societe en Commandite
InventorsHargurdeep Saini, Kumari Koonjul, Jihad Attieh
Life of this patent
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Abstract
TMT-based methods for the selection of transgenic cells and the production of transgenic organisms are described herein. The toxin used in the selection methods is a TMT substrate. Also described herein are the recombinant cells, plants and progeny and seeds thereof having the TMT gene, a selectable marker system based on the TMT gene, and commercial packages relating to a TMT-based selectable marker system.
Claims29
A method for selecting a first cell from a population of cells, said method comprising:
- (a) providing said population of cells comprising said first cell, wherein said first cell comprises a first nucleic acid which encodes a polypeptide having TMT activity;
- (b) contacting said population of cells with a toxin, wherein said toxin is a TMT substrate;
- and
- (c) selecting said first cell by virtue of its increased tolerance to said toxin.
(canceled)
8-16. (canceled)
19-36. (canceled)
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42-44. (canceled)
A method for selecting a first plant or part thereof from a population of plants or parts thereof, said method comprising:
- (a) providing said population of plants or parts thereof comprising said first plant or part thereof, wherein said first plant or part thereof comprises a first nucleic acid which encodes a polypeptide having TMT activity;
- (b) contacting said population of plants or parts thereof with a toxin, wherein said toxin is a TMT substrate;
- and
- (c) selecting said first plant or part thereof by virtue of its increased tolerance to said toxin.
46-50. (canceled)
A plant cell comprising a recombinant vector comprising a first nucleic acid which encodes a polypeptide having TMT activity.
54-64. (canceled)
A selectable marker system for the selection of a first cell from a population of cells comprising:
- (a) a recombinant vector comprising a first nucleic acid which encodes a polypeptide having TMT activity;
- and
- (b) a toxin, wherein said toxin is a TMT substrate.
68-70. (canceled)
A commercial package comprising:
- (a) a recombinant vector comprising a nucleic acid encoding a polypeptide having TMT activity;
- and
- (b) instructions for the selection of a first cell from a population of cells.
72-76. (canceled)
Dependencies are inferred from the claim text.